A red-shifted fluorescent substrate for aldehyde dehydrogenase

被引：0
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作者：
Il Minn
Haofan Wang
Ronnie C. Mease
Youngjoo Byun
Xing Yang
Julia Wang
Steven D. Leach
Martin G. Pomper
机构：
[1] Russel H. Morgan Department of Radiology and Radiological Science,
[2] College of Pharmacy,undefined
[3] Korea University,undefined
[4] 2511 Sejong-ro,undefined
[5] Jochiwon-eup,undefined
[6] Sejong 339-700,undefined
[7] South Korea,undefined
[8] Department of Surgery and the McKusick-Nathans Institute of Genetic Medicine,undefined
[9] Sidney Kimmel Comprehensive Cancer Center,undefined
[10] Johns Hopkins University,undefined
来源：
Nature Communications | / 5卷
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摘要：
Selection of cells positive for aldehyde dehydrogenase (ALDH) activity from a green-fluorescent background is difficult with existing reagents. Here we report a red-shifted fluorescent substrate for ALDH, AldeRed 588-A, for labelling viable ALDHpos cells. We demonstrate that AldeRed 588-A successfully isolates ALDHhi human haematopoietic stem cells from heterogeneous cord blood mononuclear cells. AldeRed 588-A can be used for multicolour applications to fractionate ALDHpos cells in the presence of green fluorophores including the ALDEFLUOR reagent and cells expressing enhanced green-fluorescent protein (eGFP). AldeRed 588-A stains ALDHpos murine pancreatic centroacinar and terminal duct cells, as visualized using fluorescent microscopy. AldeRed588-A provides a useful tool to select stem cells or study ALDH within a green-fluorescent background.
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