Isolation and characterization of microsatellite loci in Onychostoma sima

被引：0
|
作者：
Meihua Xiong
Yanfu Que
Fang Shi
Nian Xu
Zhong Yang
Jianbo Chang
Juxiang Hu
Bin Zhu
机构：
[1] Ministry of Water Resources and Chinese Academy of Sciences,Institute of Hydroecology
来源：
Conservation Genetics Resources | 2009年 / 1卷
关键词：
 ; Microsatellite; Polymorphism;
D O I：
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中图分类号：
学科分类号：
摘要：
Seventeen polymorphic microsatellite loci have been isolated from an (AAAG)n enriched genomic library of Onychostoma sima. These loci were tested against 32 wild captured individuals for polymorphism. The number of observed alleles per locus ranged from 2 to 10. The expected and observed heterozygosity values ranged from 0.25 to 0.96 and from 0.26 to 0.87, respectively. Seven loci showed significant deviation from Hardy–Weinberg Equilibrium and linkage equilibrium were detected in three loci. These polymorphic loci described for O. sima will be valuable as genetic markers for use in population structure, breeding, and conservation studies.
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