Simultaneous co-immobilization of glucose oxidase and catalase in their substrates

被引：0
|
作者：
G. Ozyilmaz
S. S. Tukel
机构：
[1] University of Mustafa Kemal,Faculty of Arts and Sciences Department of Chemistry
[2] University of Cukurova,Faculty of Arts and Sciences Department of Chemistry
来源：
Applied Biochemistry and Microbiology | 2007年 / 43卷
关键词：
Immobilization; Apply Biochemistry; Glucose Oxidase; Gluconic Acid; Florisil;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Glucose oxidase (GOD) and catalase (CAT) were simultaneously co-immobilized onto magnesium silicate (Florisil®) by covalent coupling. Glucose was added in immobilization mixture and hydrogen peroxide, which is the substrate of CAT, was produced in coupling mixture during immobilization time. Therefore, co-immobilization of GOD and CAT was carried out in the presence of both their substrates: glucose and hydrogen peroxide, respectively. The effect of glucose concentration in immobilization mixture on activities of GOD and CAT of co-immobilized samples were investigated. Maximum GOD and CAT activities were determined for samples co-immobilized in the presence of 15 and 20 mM glucose, respectively. Co-immobilization of GOD and CAT in the presence of their substrates highly improved the activity and reusability of both enzymes.
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页码：29 / 35
页数：6
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