Identification of a novel fatty acid elongase with a wide substrate specificity from arachidonic acid-producing fungus Mortierella alpina 1S-4

被引:0
|
作者
Eiji Sakuradani
Masutoshi Nojiri
Haruna Suzuki
Sakayu Shimizu
机构
[1] Kyoto University,Division of Applied Life Sciences, Graduate School of Agriculture
来源
Applied Microbiology and Biotechnology | 2009年 / 84卷
关键词
Fatty acid elongase; RNA interference; Gene expression; Polyunsaturated fatty acid;
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学科分类号
摘要
The isolation and characterization of a gene (MALCE1) that encodes a fatty acid elongase from arachidonic acid-producing fungus Mortierella alpina 1S-4 are described. MALCE1 was confirmed to encode a fatty acid elongase by its expression in yeast Saccharomyces cerevisiae, resulting in the accumulation of 18-, 19-, and 20-carbon monounsaturated fatty acids and eicosanoic acid. Furthermore, the MALCE1 yeast transformant efficiently elongated exogenous 9-hexadecenoic acid, 9,12-octadecadienoic acid, and 9,12,15-octadecatrienoic acid. The MALCE1 gene-silenced strain obtained from M. alpina 1S-4 exhibited a low content of octadecanoic acid and a high content of hexadecanoic acid, compared with those in the wild strain. The enzyme encoded by MALCE1 was demonstrated to be involved in the conversion of hexadecanoic acid to octadecanoic acid, its main role in M. alpina 1S-4.
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页码:709 / 716
页数:7
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