Design of a PCR test based on the gyrA gene sequence for the identification of closely related species of the Bacillus subtilis group

被引：0
|
作者：
L. N. Borshchevskaya
A. N. Kalinina
S. P. Sineokii
机构：
[1] State Research Institute for Genetics and Selection of Industrial Microorganisms (GosNIIgenetika),
来源：
Applied Biochemistry and Microbiology | 2013年 / 49卷
关键词：
gene; identification; species-specific primers;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A method for the taxonomic identification of seven closely related bacterial species of the Bacillus subtilis group (B. subtilis, B. amyloliquefaciens, B. licheniformis, B. vallismortis, B. atrophaeus, B. sonorensis, and B. mojavensis) using specific primers selected on the basis of the gyrA gene sequences was developed. The effectiveness of this method both for the identification of pure cultures of type strains of this group and for the precise species identification of collection and industrial bacterial strains was demonstrated. The principal possibility of using this method for detecting B. subtilis group bacteria in mixed cultures was shown.
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页码：646 / 655
页数：9
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