Development and characterisation of highly antibiotic resistant Bartonella bacilliformis mutants

被引:0
|
作者
Cláudia Gomes
Sandra Martínez-Puchol
Lidia Ruiz-Roldán
Maria J. Pons
Juana del Valle Mendoza
Joaquim Ruiz
机构
[1] ISGlobal,
[2] Barcelona Ctr. Int. Health Res. (CRESIB),undefined
[3] Hospital Clínic - Universitat de Barcelona,undefined
[4] School of Medicine,undefined
[5] Research Center and Innovation of the Health Sciences Faculty,undefined
[6] Universidad Peruana de Ciencias Aplicadas (UPC),undefined
[7] Instituto de Investigación Nutricional,undefined
[8] Present address: Laboratory of Virus Contaminants of Water and Food,undefined
[9] Department of Microbiology,undefined
[10] University of Barcelona,undefined
[11] Barcelona,undefined
[12] Catalonia,undefined
[13] Spain.,undefined
[14] Present address: Molecular Microbiology area,undefined
[15] Center for Biomedical Research of La Rioja,undefined
[16] Logroño,undefined
[17] Spain.,undefined
来源
Scientific Reports | / 6卷
关键词
D O I
暂无
中图分类号
学科分类号
摘要
The objective was to develop and characterise in vitro Bartonella bacilliformis antibiotic resistant mutants. Three B. bacilliformis strains were plated 35 or 40 times with azithromycin, chloramphenicol, ciprofloxacin or rifampicin discs. Resistance-stability was assessed performing 5 serial passages without antibiotic pressure. MICs were determined with/without Phe-Arg-β-Napthylamide and artesunate. Target alterations were screened in the 23S rRNA, rplD, rplV, gyrA, gyrB, parC, parE and rpoB genes. Chloramphenicol and ciprofloxacin resistance were the most difficult and easiest (>37.3 and 10.6 passages) to be selected, respectively. All mutants but one selected with chloramphenicol achieved high resistance levels. All rifampicin, one azithromycin and one ciprofloxacin mutants did not totally revert when cultured without antibiotic pressure. Azithromycin resistance was related to L4 substitutions Gln-66 → Lys or Gly-70 → Arg; L4 deletion Δ62–65 (Lys-Met-Tyr-Lys) or L22 insertion 83::Val-Ser-Glu-Ala-His-Val-Gly-Lys-Ser; in two chloramphenicol-resistant mutants the 23S rRNA mutation G2372A was detected. GyrA Ala-91 → Val and Asp-95 → Gly and GyrB Glu474 → Lys were detected in ciprofloxacin-resistant mutants. RpoB substitutions Gln-527 → Arg, His-540 → Tyr and Ser-545 → Phe plus Ser-588 → Tyr were detected in rifampicin-resistant mutants. In 5 mutants the effect of efflux pumps on resistance was observed. Antibiotic resistance was mainly related to target mutations and overexpression of efflux pumps, which might underlie microbiological failures during treatments.
引用
收藏
相关论文
共 50 条
  • [41] Characterisation of in vitro-selected mutants of Ureaplasma parvum resistant to macrolides and related antibiotics
    Pereyre, S.
    Metifiot, M.
    Cazanave, C.
    Renaudin, H.
    Charron, A.
    Bebear, C.
    Bebear, C. M.
    INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2007, 29 (02) : 207 - 211
  • [42] Characterisation of oxidative stress-resistant mutants of Streptococcus thermophilus CNRZ368
    Fernandez, A
    Thibessard, A
    Borges, F
    Gintz, B
    Decaris, B
    Leblond-Bourget, N
    ARCHIVES OF MICROBIOLOGY, 2004, 182 (05) : 364 - 372
  • [43] Technological and probiotic characterisation of Lactobacillus casei/paracasei strains and their phage-resistant mutants
    Capra, Maria L.
    Tibaldo, Maximiliano M.
    Vinderola, Gabriel
    Reinheimer, Jorge A.
    Quiberoni, Andrea
    INTERNATIONAL DAIRY JOURNAL, 2014, 37 (01) : 39 - 47
  • [44] Frequency and characterisation of spontaneous lipiarmycin-resistant Enterococcus faecalis mutants selected in vitro
    Gualtieri, Maxime
    Tupin, Audrey
    Brodolin, Konstantin
    Leonetti, Jean-Paul
    INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2009, 34 (06) : 605 - 606
  • [45] Characterisation of oxidative stress-resistant mutants of Streptococcus thermophilus CNRZ368
    Annabelle Fernandez
    Annabelle Thibessard
    Frédéric Borges
    Brigitte Gintz
    Bernard Decaris
    Nathalie Leblond–Bourget
    Archives of Microbiology, 2004, 182 : 364 - 372
  • [46] Characterisation of p-nitrophenylglycerol-resistant Proteus mirabilis super-swarming mutants
    Liaw, SJ
    Lai, HC
    Ho, SW
    Luh, KT
    Wang, WB
    JOURNAL OF MEDICAL MICROBIOLOGY, 2001, 50 (12) : 1039 - 1048
  • [47] Characterisation of Ramularia collo-cygni laboratory mutants resistant to succinate dehydrogenase inhibitors
    Piotrowska, Marta J.
    Fountaine, James M.
    Ennos, Richard A.
    Kaczmarek, Maciej
    Burnett, Fiona J.
    PEST MANAGEMENT SCIENCE, 2017, 73 (06) : 1187 - 1196
  • [48] EFFICIENT INDUCTION AND SELECTION OF CHLOROPLAST-ENCODED ANTIBIOTIC-RESISTANT MUTANTS IN NICOTIANA
    FLUHR, R
    AVIV, D
    GALUN, E
    EDELMAN, M
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (05) : 1485 - 1489
  • [49] Rapid and robust evolution of collateral sensitivity in Pseudomonas aeruginosa antibiotic-resistant mutants
    Hernando-Amado, Sara
    Sanz-Garcia, Fernando
    Luis Martinez, Jose
    SCIENCE ADVANCES, 2020, 6 (32)
  • [50] ISOLATION AND GENETIC-CHARACTERIZATION OF ANTIBIOTIC-RESISTANT MUTANTS OF CHLAMYDOMONAS-REINHARDII
    DORSEY, MJ
    OHIO JOURNAL OF SCIENCE, 1987, 87 (02) : 32 - 32