Complete genome sequences of a putative new alphapartitivirus detected in Rosa spp.

被引:0
|
作者
James Phelan
Delano James
机构
[1] Canadian Food Inspection Agency,Sidney Laboratory
来源
Archives of Virology | 2016年 / 161卷
关键词
Sprit; Accession Number KU896858; dsRNA2 Fragment; dsRNA Extraction; Cultivar Antique;
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学科分类号
摘要
A putative new alphapartitivirus was detected by next-generation sequencing (NGS) in Rosa spp. and identified as rose partitivirus isolate Phyllis Bide (RoPV-PB). The virus is bipartite with a dsRNA1 fragment (1937 bp) encoding a putative RdRp and a dsRNA2 fragment (1811 bp) encoding the putative CP subunit of the virus. dsRNA1 of RoPV-BP is closely related to Vicia faba partitivirus 1, with identities of 67 % and 72 % for the nucleotide (nt) and deduced amino acid (aa) sequences, respectively. In NGS analysis of RoPV-BP, coverage was uneven across both dsRNA fragments, with GC/AT content appearing to be a major determinant of depth of coverage.
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页码:2623 / 2626
页数:3
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