Effect of Genotype and Explant Age on Callus Induction and Subsequent Plant Regeneration from Root-derived Callus of Indica Rice Genotypes

被引:0
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作者
Md. Enamul Hoque
John W. Mansfield
机构
[1] Bangladesh Rice Research Institute,Biotechnology Division
[2] Imperial College London,Department of Agriculture Sciences
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关键词
callus induction; genotypic variations; Indica rice; plant regeneration; root segments;
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摘要
An attempt has been taken to establish an efficient plant regeneration system in vitro from 3, 5, 7 and 9-days-old root segments of four Indica (Bangladeshi) rice genotypes. Genotypic effects were observed in callus induction and subsequent plant regeneration. Moreover, the stage of development of the root explants also played a significant role in callus formation and subsequent plant regeneration. Younger explants were more efficient in both callus induction and plant regeneration. Plants regenerated in vitro were successfully established in soil and produced fertile seeds.
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页码:217 / 223
页数:6
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