Duplex real-time PCR for determining zygosity in transgenic plants [Duplex-Real-Time-PCR zur Zygositäts-bestimmung in transgenen Pflanzen]

被引：0

作者：

Fritsch L.D. ^{[1
]}

Schillberg S. ^{[1
,2
]}

Schröper F. ^{[1
,3
]}

机构：

[1] Fraunhofer-Institut für Molekularbiologie und Angewandte Oekologie IME, Aachen

[2] Abteilung Phytopathologie, Institut für Phytopathologie und Angewandte Zoologie, Universität Gießen

[3] Fraunhofer-Institut für Molekularbiologie und Angewandte Oekologie IME, Forckenbeckstraße 6, Aachen

来源：

BIOspektrum | 2015年 / 21卷 / 5期

关键词：

D O I：

10.1007/s12268-015-0611-x

中图分类号：

学科分类号：

摘要：

Reliable techniques to detect the presence and zygosity of transgenic events in plants are crucial for biotechnology research and plant breeding industry. Therefore, we have introduced a novel assay, which enables high-throughput genotyping of transgenic events in maize using duplex real-time PCR. We demonstrated that the presence and zygosity of two selected transgenic events could be detected with high reproducibility and that our method is suitable for rapid genotyping of a large number of samples. © 2015, Springer-Verlag Berlin Heidelberg.

引用

页码：511 / 514

页数：3

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