Duplex real-time PCR for determining zygosity in transgenic plants [Duplex-Real-Time-PCR zur Zygositäts-bestimmung in transgenen Pflanzen]

Reliable techniques to detect the presence and zygosity of transgenic events in plants are crucial for biotechnology research and plant breeding industry. Therefore, we have introduced a novel assay, which enables high-throughput genotyping of transgenic events in maize using duplex real-time PCR. We demonstrated that the presence and zygosity of two selected transgenic events could be detected with high reproducibility and that our method is suitable for rapid genotyping of a large number of samples. © 2015, Springer-Verlag Berlin Heidelberg.