PpRab1, a Rab GTPase from maritime pine is differentially expressed during embryogenesis

被引:0
|
作者
Sónia Gonçalves
John Cairney
María Pérez Rodríguez
Francisco Cánovas
Margarida Oliveira
Célia Miguel
机构
[1] Instituto de Biologia Experimental e Tecnológica (IBET)/Instituto de Tecnologia Química e Biológica (ITQB),Forest Biotech Lab
[2] Georgia Institute of Technology,School of Biology and Institute of Paper Science and Technology at Georgia Tech
[3] Universidad de Málaga,Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias
[4] Univ. Lisboa,Dep. Biologia Vegetal
[5] Fac. Ciências,undefined
来源
关键词
Embryogenesis; GTP-binding protein; Rab family; Maritime pine;
D O I
暂无
中图分类号
学科分类号
摘要
Rab-related small GTP-binding proteins are known to be involved in the regulation of the vesicular transport system in eukaryotic cells. We report the characterization of a previously isolated full-length cDNA PpRab1 from Pinus pinaster. Amino acid sequence analysis revealed the presence of G1–G5 conserved domains of the GTPase Ras superfamily and a double cysteine motif in the C-terminal, characteristic of Rab proteins. The PpRab1 protein shows high sequence similarity to several Rab1 GTP-binding proteins in plants. Phylogenetic analysis showed that, within the Ras superfamily, PpRab1 is more closely related to the Rab family and within this, PpRab1 protein was found to cluster with Arabidopsis subfamily AtRABE, whose members are known to regulate ER-to-Golgi membrane trafficking steps. PpRab1 transcripts were expressed at constitutively high levels for the initial stages of zygotic embryo development, and then their relative abundance decreased as embryo matures. The PpRab1 transcript is not embryo-specific as it was found in roots, cotyledons and hypocotyls. An increase in PpRab1 expression level was observed when seeds are germinated and collected at successive time points of development. In situ RT-PCR analysis revealed an expression signal in early zygotic embryos. In view of the proposed roles of Rab1 GTP-binding protein, the possible function of the protein encoded by PpRab1 in embryogenesis is discussed.
引用
收藏
页码:273 / 282
页数:9
相关论文
共 50 条
  • [21] Identification and characterization of transcripts differentially expressed during embryogenesis in Capsella bursa-pastoris
    Tao, P.
    Wang, J. B.
    BIOLOGIA PLANTARUM, 2012, 56 (03) : 415 - 421
  • [22] Analysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinus pinaster)
    Liliana Marum
    Margarida Rocheta
    João Maroco
    M. Margarida Oliveira
    Célia Miguel
    Plant Cell Reports, 2009, 28 : 673 - 682
  • [23] Analysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinus pinaster)
    Marum, Liliana
    Rocheta, Margarida
    Maroco, Joao
    Oliveira, M. Margarida
    Miguel, Celia
    PLANT CELL REPORTS, 2009, 28 (04) : 673 - 682
  • [24] Priming Maritime Pine Megagametophytes during Somatic Embryogenesis Improved Plant Adaptation to Heat Stress
    Perez-Oliver, Maria Amparo
    Haro, Juan Gregorio
    Pavlovic, Iva
    Novak, Ondrej
    Segura, Juan
    Sales, Ester
    Arrillaga, Isabel
    PLANTS-BASEL, 2021, 10 (03): : 1 - 26
  • [25] The role of arginine metabolic pathway during embryogenesis and germination in maritime pine (Pinus pinaster Ait.)
    Llebres, Maria-Teresa
    Pascual, Maria-Belen
    Debille, Sandrine
    Trontin, Jean-Francois
    Harvengt, Luc
    Avila, Concepcion
    Canovas, Francisco M.
    TREE PHYSIOLOGY, 2018, 38 (03) : 471 - 484
  • [26] Expressed Sequence Tags from loblolly pine embryos reveal similarities with angiosperm embryogenesis
    Cairney, John
    Zheng, Li
    Cowels, Allison
    Hsiao, Joseph
    Zismann, Victoria
    Liu, Jia
    Ouyang, Shu
    Thibaud-Nissen, Francoise
    Hamilton, John
    Childs, Kevin
    Pullman, Gerald S.
    Zhang, Yiting
    Oh, Thomas
    Buell, C. Robin
    PLANT MOLECULAR BIOLOGY, 2006, 62 (4-5) : 485 - 501
  • [27] Expressed Sequence Tags from loblolly pine embryos reveal similarities with angiosperm embryogenesis
    John Cairney
    Li Zheng
    Allison Cowels
    Joseph Hsiao
    Victoria Zismann
    Jia Liu
    Shu Ouyang
    Francoise Thibaud-Nissen
    John Hamilton
    Kevin Childs
    Gerald S. Pullman
    Yiting Zhang
    Thomas Oh
    C. Robin Buell
    Plant Molecular Biology, 2006, 62 : 485 - 501
  • [28] Two follistatin-like 1 homologs are differentially expressed in adult tissues and during embryogenesis in grass carp (Ctenopharyngodon idellus)
    Sun, Yi-Wen
    Li, Fu-Gui
    Chen, Jie
    Jiang, Xia-Yun
    Zou, Shu-Ming
    GENERAL AND COMPARATIVE ENDOCRINOLOGY, 2015, 223 : 1 - 8
  • [29] Somatic embryogenesis from 20 open-pollinated families of Portuguese plus trees of maritime pine
    Miguel, C
    Gonçalves, S
    Tereso, S
    Marum, L
    Maroco, J
    Oliveira, MM
    PLANT CELL TISSUE AND ORGAN CULTURE, 2004, 76 (02) : 121 - 130
  • [30] Somatic Embryogenesis from 20 Open-Pollinated Families of Portuguese Plus Trees of Maritime Pine
    Célia Miguel
    Sónia Gonçalves
    Susana Tereso
    Liliana Marum
    João Maroco
    M. Margarida Oliveira
    Plant Cell, Tissue and Organ Culture, 2004, 76 : 121 - 130