Characteristics of airborne bacteria and fungi in some Polish wastewater treatment plants

The aim of this study was to characterize both the viable airborne bacteria and fungi collected in different types of wastewater treatment plants in Poland. Bacterial and fungal aerosols in five different wastewater treatment plants in Poland were studied, including the pioneering antibiotic resistance testing of airborne bacteria. Concentration level was measured using the six-stage Andersen impactor. After incubation, bioaerosol particles captured on nutrient media on Petri dishes were quantitatively evaluated and qualitatively identified. It was found that the concentration levels of both airborne bacteria and fungi ranged from 10² to 10³ colony-forming units/m³, in all stages of the wastewater treatment plants. The patterns of size distributions of airborne bacteria and fungi were very similar, showing dominant peaks typically in the size range between 3.3 and 4.7 μm. The majority fraction of small bacterial and fungal particles (less than 4.7 μm) in the studied areas indicates that the bioaerosol is relatively fresh, and mostly of local origin. The Gram-positive cocci and nonsporing Gram-positive rods were the dominating forms in the studied wastewater treatment plants environment. The existing differences in the concentration levels between sampling sites enabled identification of the main bioaerosol sources. The highest concentration of bacterial aerosol appeared in sections, where activated sludge post-processing and mechanical purifying are conducted. The most frequently occurring species in the sampled bacterial aerosol were Gram-positive cocci and nonsporing Gram-positive rods. Multi-antibiotic resistance testing showed that among the isolated airborne bacteria, the most antibiotic-resistant features were present among Bacillus species (especially Bacillus mycoides).