Synthesis of a highly Mg2+-selective fluorescent probe and its application to quantifying and imaging total intracellular magnesium

被引:0
|
作者
Azzurra Sargenti
Giovanna Farruggia
Nelsi Zaccheroni
Chiara Marraccini
Massimo Sgarzi
Concettina Cappadone
Emil Malucelli
Alessandra Procopio
Luca Prodi
Marco Lombardo
Stefano Iotti
机构
[1] University of Bologna,Department of Pharmacy and Biotechnologies
[2] National Institute of Biostructures and Biosystems,Department of Chemistry ′Giacomo Ciamician’
[3] University of Bologna,Department of Transfusion Medicine
[4] ASMN-IRCCS,undefined
来源
Nature Protocols | 2017年 / 12卷
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摘要
Specific and sensitive tools for quantifying intracellular magnesium cations are needed in biochemical research. This protocol describes the synthesis and use of a fluorescent Mg2+ probe based on diaza-18-crown-6-hydroxyquinoline.
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页码:461 / 471
页数:10
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