A highly sensitive and selective fluorescent probe for Hg2+ and its imaging application in living cells

被引:18
|
作者
Xu, Zhi-Hong [1 ,2 ]
Hou, Xu-Feng [1 ]
Xu, Wen-Lei [1 ]
Guo, Rui [1 ,2 ]
Xiang, Tian-Cheng [1 ]
机构
[1] Xuchang Univ, Sch Chem & Chem Engn, Xuchang 461000, Peoples R China
[2] Xuchang Univ, Inst Surface Micro & Nano Mat, Key Lab Micronano Mat Energy Storage & Convers He, Xuchang 461000, Peoples R China
关键词
Fluorescent probe; Rhodamine derivatives; Hg2+; Imaging application; TURN-ON; AQUEOUS-SOLUTION; RHODAMINE; SENSOR; MERCURY; CHEMODOSIMETER; IONS; CHEMOSENSOR; CU2+; RECEPTOR;
D O I
10.1016/j.inoche.2013.05.008
中图分类号
O61 [无机化学];
学科分类号
070301 ; 081704 ;
摘要
Two rhodamine-based Hg2+ probes L1 and L2 were prepared and confirmed by NMR, MS and crystal data. The sensing behavior of these probes was studied by UV-visible and fluorescence spectroscopy. The probe L1 couldn't be widely applied in aqueous media or mixed media because of its poor solubility. However, the probe 12 displayed selective and sensitive fluorescence enhancement response to Hg2+ at physiological pH value for biological use. The selectivity of this system for Hg2+ over other metal ions was remarkably high, and its sensitivity was at ppb level. The probe L2 responded rapidly to Hg2+ in aqueous solutions with a 1:1 stoichiometry and was successfully applied to image Hg2+ in living cells. (c) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:42 / 46
页数:5
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