Rapid autopsies to enhance metastatic research: the UPTIDER post-mortem tissue donation program

被引：0

作者：

Geukens, Tatjana ^{[1
,2
]}

De Schepper, Maxim ^{[1
,3
]}

van den Bogaert, Wouter ^{[4
]}

Van Baelen, Karen ^{[1
,5
]}

Maetens, Marion ^{[1
]}

Pabba, Anirudh ^{[1
]}

Mahdami, Amena ^{[1
]}

Leduc, Sophia ^{[1
]}

Isnaldi, Edoardo ^{[1
]}

Nguyen, Ha-Linh ^{[1
]}

Bachir, Imane ^{[1
,6
]}

Hajipirloo, Maysam ^{[1
]}

Zels, Gitte ^{[1
,3
]}

Van Cauwenberge, Josephine ^{[1
,5
]}

Borremans, Kristien ^{[1
,5
]}

Vandecaveye, Vincent ^{[7
]}

Weynand, Birgit ^{[3
]}

Vermeulen, Peter ^{[8
]}

Leucci, Eleonora ^{[9
]}

Baietti, Maria Francesca ^{[9
]}

Sflomos, George ^{[10
]}

Battista, Laura ^{[10
]}

Brisken, Cathrin ^{[10
,11
]}

Derksen, Patrick W. B. ^{[12
]}

Koorman, Thijs ^{[12
]}

Visser, Daan ^{[12
]}

Scheele, Colinda L. G. J. ^{[13
]}

Thommen, Daniela S. ^{[14
]}

Hatse, Sigrid ^{[15
]}

Fendt, Sarah-Maria ^{[16
,17
,18
]}

Vanderheyden, Evy ^{[19
,20
]}

Van Brussel, Thomas ^{[19
,20
]}

Schepers, Rogier ^{[19
,20
]}

Boeckx, Bram ^{[19
,20
]}

Lambrechts, Diether ^{[19
,20
]}

Marano, Giuseppe ^{[21
,22
]}

Biganzoli, Elia ^{[21
,22
]}

Smeets, Ann ^{[23
]}

Nevelsteen, Ines ^{[23
]}

Punie, Kevin ^{[2
]}

Neven, Patrick ^{[5
]}

Wildiers, Hans ^{[2
]}

Richard, Francois ^{[1
]}

Floris, Giuseppe ^{[3
]}

Desmedt, Christine ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Dept Oncol, Lab Translat Breast Canc Res, Leuven, Belgium

[2] Univ Hosp Leuven, Dept Gen Med Oncol, Leuven, Belgium

[3] Univ Hosp Leuven, Dept Pathol, Leuven, Belgium

[4] Univ Hosp Leuven, Dept Forens Med, Leuven, Belgium

[5] Univ Hosp Leuven, Dept Gynecol & Obstet, Leuven, Belgium

[6] Inst Jules Bordet, Dept Anesthesiol, Brussels, Belgium

[7] Univ Hosp Leuven, Dept Radiol, Leuven, Belgium

[8] Univ Antwerp, Ctr Oncol Res CORE, Antwerp, Belgium

[9] Katholieke Univ Leuven, Dept Oncol, TRACE & Lab RNA Canc Biol, Leuven, Belgium

[10] Ecole Polytech Fed Lausanne EPFL, ISREC Swiss Inst Expt Canc Res, Sch Life Sci, Lausanne, Switzerland

[11] Inst Canc Res, Breast Canc Now Toby Robins Breast Canc Res Ctr, London, England

[12] Univ Med Ctr, Dept Pathol, Utrecht, Netherlands

[13] VIB KU Leuven Ctr Canc Biol, Dept Oncol, Lab Intravital Microscopy & Dynam Tumor Progress, Leuven, Belgium

[14] Netherlands Canc Inst, Div Mol Oncol & Immunol, Amsterdam, Netherlands

[15] Dept Oncol, Lab Expt Oncol, Dept Oncol, Leuven, Belgium

[16] VIB KU Leuven Ctr Canc Biol, Lab Cellular Metab & Metab Regulat, Leuven, Belgium

[17] Dept Oncol, Lab Cellular Metab & Metab Regulat, KU Leuven, Leuven, Belgium

[18] Leuven Canc Inst LKI, Leuven, Belgium

[19] Dept Human Genet, Lab Translat Genet, KU Leuven, Leuven, Belgium

[20] VIB Ctr Canc Biol, Leuven, Belgium

[21] Univ Milan, Dept Biomed & Clin Sci DIBIC L Sacco, Unit Med Stat Biometry & Epidemiol, LITA Vialba Campus, Milan, Italy

[22] Univ Milan, DSRC, LITA Vialba Campus, Milan, Italy

[23] Univ Hosp Leuven, Dept Surg Oncol, Leuven, Belgium

来源：

NPJ BREAST CANCER | 2024年 / 10卷 / 01期

基金：

欧盟地平线“2020”; 欧洲研究理事会;

关键词：

PROGESTERONE-RECEPTOR; GENE-EXPRESSION; PRIMARY TUMORS; COLD ISCHEMIA; BREAST; HETEROGENEITY; PROCUREMENT; ESTROGEN; MODEL; TIME;

D O I：

10.1038/s41523-024-00637-3

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Research on metastatic cancer has been hampered by limited sample availability. Here we present the breast cancer post-mortem tissue donation program UPTIDER and show how it enabled sampling of a median of 31 (range: 5-90) metastases and 5-8 liquids per patient from its first 20 patients. In a dedicated experiment, we show the mild impact of increasing time after death on RNA quality, transcriptional profiles and immunohistochemical staining in tumor tissue samples. We show that this impact can be counteracted by organ cooling. We successfully generated ex vivo models from tissue and liquid biopsies from distinct histological subtypes of breast cancer. We anticipate these and future findings of UPTIDER to elucidate mechanisms of disease progression and treatment resistance and to provide tools for the exploration of precision medicine strategies in the metastatic setting.

引用

页数：14

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