Resistance of the cell wall to degradation is a critical parameter for isolation of high quality RNA from natural isolates of Bacillus subtilis

被引：0
|
作者：
Jean-Sébastien Guez
François Coutte
Anne-Sophie Drucbert
Nour-Eddine Chihib
Pierre-Marie Danzé
Philippe Jacques
机构：
[1] Université des Sciences et Technologies de Lille,Laboratoire ProBioGEM, UPRES
[2] IFR114-IMPRT (functional genomic platform),EA 1026, Polytech
来源：
Archives of Microbiology | 2009年 / 191卷
关键词：
RNA isolation; Natural strain; Cell wall; Transcript analysis; RT-PCR;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Natural isolates of Bacillus subtilis are known for their ability to produce a large panel of bioactive compounds. Unfortunately, their recalcitrance to conventional molecular techniques limits their transcript studies. In this work, difficulties to isolate RNA attributed to the cell wall were overcome, finally authorising powerful RT-PCR’s.
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页码：669 / 673
页数：4
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