Extraction of high-quality RNA from Bacillus subtilis with a lysozyme pre-treatment followed by the Trizol method

被引：36
|
作者：
Eber, Villa-Rodriguez [1 ]
Cuauhtemoc, Ibarra-Gamez [1 ]
Sergio, de los Santos-Villalobos [2 ]
机构：
[1] Inst Tecnol Sonora, 5 Febrero 818 Sur, Obregon 85000, Sonora, Mexico
[2] Inst Tecnol Sonora, CONACYT, 5 Febrero 818 Sur, Obregon 85000, Sonora, Mexico
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2018年 / 147卷
关键词：
RNA extraction; Bacillus subtilis; Trizol; RNA Integrity Number (RIN); INTEGRITY; BACTERIA;
D O I：
10.1016/j.mimet.2018.02.011
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A suitable technique (lysozyme combined with Trizol reagent) was developed to improve the RNA yield, purity and integrity from Bacillus subtilis, under different bacterial growth stages. The obtained RNA was intact, having the required characteristics for downstream applications.
引用
收藏
页码：14 / 16
页数：3
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