Production of L-Malic Acid by Permeabilized Cells of Commercial Saccharomyces Sp. Strains

被引：0
|
作者：
Ana Vrsalović Presečki
Đurđa Vasić-Rački
机构：
[1] University of Zagreb,Faculty of Chemical Engineering and Technology
来源：
Biotechnology Letters | 2005年 / 27卷
关键词：
fumarase; malic acid; modeling; permeabilization; yeast;
D O I：
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中图分类号：
学科分类号：
摘要：
Of various yeasts tested in the conversion of fumaric to l-malic acid, Saccharomyces bayanus had the highest activity of fumarase. Cells permeabilized with 0.2% (w/v) CTAB for 5 min gave maximum enzyme activity. Under non-growth conditions, fumarase activity in the permeabilized cells was four times higher (271 U/g) than that of the intact cells (67 U/g). The proposed mathematical model for the batch production of l-malic acid was validated at different initial fumaric acid concentrations. The average conversion of fumaric acid was up to 82% and gave 21, 40, 83 and 175 mml-malic acid from respectively, 25, 50, 100 and 210 mm fumaric acid.
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页码：1835 / 1839
页数：4
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