Determination of Polysaccharides and Lipopolysaccharides by Spectrophotometry and Thermal-Lens Spectrometry

被引：0
|
作者：
N. V. Orlova
M. A. Proskurnin
V. A. Samburova
P. V. Tsvetkov
机构：
[1] Moscow State University,Department of Chemistry
来源：
Journal of Analytical Chemistry | 2003年 / 58卷
关键词：
Analytical Chemistry; Phenol; Detection Limit; Heparin; Polysaccharide;
D O I：
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学科分类号：
摘要：
A procedure for the photometric determination of polysaccharides with phenol in a sulfuric acid solution was optimized to reduce the blank signal. The procedure was used for the thermal-lens determination of polysaccharides (heparin, dextran, plantaglucide) qualitatively reproducing the chemical properties of pyrogenic lipopolysaccharides under experimental conditions. The detection limits were found to be 2–5 μg/mL. With the use of thermal-lens spectrometry, lipopolysaccharides can be determined in water (in a model sample, pharmaceutical preparation pyrogenal) with a detection limit of 0.3 μg/mL, which is ten times lower than the minimal injected concentration of the preparation.
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页码：149 / 151
页数：2
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