Determination of polysaccharides and lipopolysaccharides by spectrophotometry and thermal-lens spectrometry

被引：8
|
作者：
Orlova, NV [1 ]
Proskurnin, MA [1 ]
Samburova, VA [1 ]
Tsvetkov, PV [1 ]
机构：
[1] Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119899, Russia
来源：
JOURNAL OF ANALYTICAL CHEMISTRY | 2003年 / 58卷 / 02期
关键词：
Analytical Chemistry; Phenol; Detection Limit; Heparin; Polysaccharide;
D O I：
10.1023/A:1022306121359
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A procedure for the photometric determination of polysaccharides with phenol in a sulfuric acid solution was optimized to reduce the blank signal. The procedure was used for the thermal-lens determination of polysaccharides (heparin, dextran, plantaglucide) qualitatively reproducing the chemical properties of pyrogenic lipopolysaccharides under experimental conditions. The detection limits were found to be 2-5 mug/mL. With the use of thermal-lens spectrometry, lipopolysaccharides can be determined in water (in a model sample, pharmaceutical preparation pyrogenal) with a detection limit of 0.3 mug/mL, which is ten times lower than the minimal injected concentration of the preparation.
引用
收藏
页码：149 / 151
页数：3
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