Increasing ethanol titer and yield in a gpd1Δ gpd2Δ strain by simultaneous overexpression of GLT1 and STL1 in Saccharomyces cerevisiae

被引：0
|
作者：
Jingyu Wang
Wen Liu
Wentao Ding
Guochang Zhang
Jingjing Liu
机构：
[1] Tianjin University,Key Laboratory of Systems Bioengineering of the Ministry of Education, Department of Biochemical Engineering, School of Chemical Engineering and Technology
来源：
Biotechnology Letters | 2013年 / 35卷
关键词：
Ethanol fermentation; Δ ; Δ; Osmotic stress;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We have investigated whether simultaneous modification of cofactor metabolism and glycerol in a strain of Saccharomyces cerevisiae can eliminate glycerol synthesis during ethanol production. Two strains, S812 (gpd1Δ gpd2Δ PGK1p-GLT1) and LE17 (gpd1Δ gpd2Δ PGK1p-GLT1 PGKp-STL1) were generated that showed a 8 and 8.2 % increase in the ethanol yield, respectively, compared to the wild type KAM-2 strain. The ethanol titer was improved from 90.4 g/l for KAM-2 to 97.6 g/l for S812 and 97.8 g/l for LE17, respectively. These results provide a new insight into rationalization of metabolic engineering strategies for improvement of ethanol yield through elimination of glycerol production.
引用
收藏
页码：1859 / 1864
页数：5
相关论文
共 45 条
  • [31] Identification of regulatory elements in the Saccharomyces cerevisiae GPD1 promoter with distinct function during adaptation to or steady-state growth in NaCl.
    Alipour, H
    Eriksson, P
    Blomberg, A
    YEAST, 2003, 20 : S201 - S201
  • [32] Ethanol yield improvement in Saccharomyces cerevisiae GPD2 Delta FPS1 Delta ADH2 Delta DLD3 Delta mutant and molecular mechanism exploration based on the metabolic flux and transcriptomics approaches
    Yang, Peizhou
    Jiang, Shuying
    Lu, Shuhua
    Jiang, Suwei
    Jiang, Shaotong
    Deng, Yanhong
    Lu, Jiuling
    Wang, Hu
    Zhou, Yong
    MICROBIAL CELL FACTORIES, 2022, 21 (01)
  • [33] Ethanol yield improvement in Saccharomyces cerevisiae GPD2 Delta FPS1 Delta ADH2 Delta DLD3 Delta mutant and molecular mechanism exploration based on the metabolic flux and transcriptomics approaches
    Peizhou Yang
    Shuying Jiang
    Shuhua Lu
    Suwei Jiang
    Shaotong Jiang
    Yanhong Deng
    Jiuling Lu
    Hu Wang
    Yong Zhou
    Microbial Cell Factories, 21
  • [34] Expression of GPD1 and SIP18 genes during rehydration in active dry industrial Saccharomyces cerevisiae cider-making yeast strains (ADY)
    Goncerzewicz, Anna
    Kaminska-Wojteczek, Karolina
    Mlynarczyk, Izabella
    Misiewicz, Anna
    ACTA BIOCHIMICA POLONICA, 2017, 64 (02) : 287 - 294
  • [35] Overexpression of PDE2 or SSD1-V in Saccharomyces cerevisiae W303-1A strain renders it ethanol-tolerant
    Avrahami-Moyal, Liat
    Braun, Sergei
    Engelberg, David
    FEMS YEAST RESEARCH, 2012, 12 (04) : 447 - 455
  • [36] GENE GPD1 ENCODING GLYCEROL-3-PHOSPHATE DEHYDROGENASE IS ESSENTIAL FOR GROWTH UNDER OSMOTIC-STRESS IN SACCHAROMYCES-CEREVISIAE AND ITS EXPRESSION IS REGULATED BY THE HOG PATHWAY
    ALBERTYN, J
    HOHMANN, S
    THEVELEIN, JM
    PRIOR, BA
    ARCHIVES INTERNATIONALES DE PHYSIOLOGIE DE BIOCHIMIE ET DE BIOPHYSIQUE, 1994, 102 (02): : B31 - B31
  • [37] GPD1, WHICH ENCODES GLYCEROL-3-PHOSPHATE DEHYDROGENASE, IS ESSENTIAL FOR GROWTH UNDER OSMOTIC-STRESS IN SACCHAROMYCES-CEREVISIAE, AND ITS EXPRESSION IS REGULATED BY THE HIGH-OSMOLARITY GLYCEROL RESPONSE PATHWAY
    ALBERTYN, J
    HOHMANN, S
    THEVELEIN, JM
    PRIOR, BA
    MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (06) : 4135 - 4144
  • [38] Simultaneous Saccharification and Ethanol Fermentation of Corn Stover at High Temperature and High Solids Loading by a Thermotolerant Strain Saccharomyces cerevisiae DQ1
    Deqiang Chu
    Jian Zhang
    Jie Bao
    BioEnergy Research, 2012, 5 : 1020 - 1026
  • [39] Simultaneous Saccharification and Ethanol Fermentation of Corn Stover at High Temperature and High Solids Loading by a Thermotolerant Strain Saccharomyces cerevisiae DQ1
    Chu, Deqiang
    Zhang, Jian
    Bao, Jie
    BIOENERGY RESEARCH, 2012, 5 (04) : 1020 - 1026
  • [40] Thermosensitivity of the Saccharomyces cerevisiae gpp1gpp2 double deletion strain can be reduced by overexpression of genes involved in cell wall maintenance
    Iwona Wojda
    Jan-Paul Bebelman
    Teresa Jakubowicz
    Marco Siderius
    Archives of Microbiology, 2007, 188 : 175 - 184
12345