A Specific DNA-Dependent DNA Polymerase Is Associated with Saccharomyces cerevisiae Telomerase

被引：0
|
作者：
A. V. Petrov
P. V. Dmitriev
K. A. Sokolov
O. A. Dontsova
机构：
[1] Lomonosov Moscow State University,School of Chemistry
[2] Russian Academy of Sciences,Engelhardt Institute of Molecular Biology
来源：
Biochemistry (Moscow) | 2001年 / 66卷
关键词：
yeast telomerase; DNA-polymerase δ; affinity tagging; telomerase reverse transcriptase;
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摘要：
The telomere DNA of most eucaryotes consists of tandem DNA repeats and a number of associated proteins. The synthesis of the G-rich DNA strand is performed by the telomerase complex. The complementary C-strand is synthesized by DNA-dependent DNA polymerases. Using telomerase reverse transcriptase tagging followed by immunoprecipitation cou pled with two-step purification, we have found a specific DNA-dependent DNA polymerase activity associated with telom erase of Saccharomyces cerevisiae. Investigation of the biochemical properties of this DNA polymerase activity confirms the hypothesis of tight interaction of DNA polymerase δ with telomerase.
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页码：1361 / 1367
页数：6
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