Osteoinduction in human fat-derived stem cells by recombinant human bone morphogenetic protein-2 produced in Escherichia coli

被引：0

作者：

Paulo C. Bessa

A. J. Pedro

B. Klösch

A. Nobre

M. van Griensven

R. L. Reis

M. Casal

机构：

[1] University of Minho,3B’s Research Group – Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering

[2] IBB – Institute for Biotechnology and Bioengineering,PT Government Associated Laboratory

[3] University of Minho,CBMA – Molecular and Environmental Biology Centre/Biology Department

[4] Ludwig Boltzmann Institute for Experimental and Clinical Traumatology,undefined

来源：

Biotechnology Letters | 2008年 / 30卷

关键词：

Bone morphogenetic protein-2; Recombinant protein purification; Stem cells; Tissue engineering;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Bioactive recombinant human bone morphogenetic protein-2 (rhBMP-2) was obtained using Escherichia coli pET-25b expression system: 55 mg purified rhBMP-2 were achieved per g cell dry wt, with up to 95% purity. In murine C2C12 cell line, rhBMP-2 induced an increase in the transcription of Smads and of osteogenic markers Runx2/Cbfa1 and Osterix, measured by semi-quantitative RT-PCR. Bioassays performed in human fat-derived stem cells showed an increased activity of the early osteogenic marker, alkaline phosphatase, and the absence of cytotoxicity.

引用

页码：15 / 21

页数：6

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