Detection and Characteristics of Rifampicin-Resistant Isolates of Mycobacterium tuberculosis

被引：0

作者：

A. G. Cherednichenko

M. A. Dymova

O. A. Solodilova

T. I. Petrenko

A. I. Prozorov

M. L. Filipenko

机构：

[1] Ministry of Health of the Russian Federation,Research Institute of Tuberculosis

[2] Siberian Division of the Russian Academy of Sciences,Institute of Chemical Biology and Fundamental Medicine

[3] Novosibirsk State University,undefined

[4] Altai Regional Antituberculous Dispensary,undefined

来源：

Bulletin of Experimental Biology and Medicine | 2016年 / 160卷

关键词：

drug resistance; rifampicin; rpoB gene;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Genotyping and analysis the drug resistance of 59 isolates of M. tuberculosis obtained from patients living in Altai Territory were performed using a BACTEC MGIT 960 fluorometric system by means of VNTR typing (variable number tandem repeat), PCR-RFLP analysis, and sequence analysis. The occurrence frequency was highest for isolates of the Beijing family (n=30, 50.8%). Analysis of mutation spectrum in the rpoB gene associated with rifampicin resistance revealed the major mutation (codon 531 of the rpoB gene) in 93% samples, which allows us to use rapid test systems.

引用

页码：659 / 663

页数：4

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