Green Synthesized Silver Nanoparticles-Mediated Cytotoxic Effect in Colorectal Cancer Cells: NF-κB Signal Induced Apoptosis Through Autophagy

被引:0
|
作者
Mahmuda Akter
A. K. M. Atique Ullah
Subrata Banik
Md. Tajuddin Sikder
Toshiyuki Hosokawa
Takeshi Saito
Masaaki Kurasaki
机构
[1] Hokkaido University,Group of Environmental Adaptation Science, Faculty of Environmental Earth Sciences
[2] Bangladesh Atomic Energy Commission,Nanoscience and Technology Research Laboratory, Chemistry Division, Atomic Energy Centre
[3] Hokkaido University,Graduate School of Environmental Science
[4] Jahangirnagar University,Department of Public Health and Informatics
[5] Hokkaido University,Research Division of Higher Education, Institute for the Advancement of Higher Education
[6] Hokkaido University,Faculty of Health Sciences
来源
Biological Trace Element Research | 2021年 / 199卷
关键词
Apoptosis; Autophagy; Brassica silver nanoparticles; NFκB; Caco-2 cells;
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中图分类号
学科分类号
摘要
Green synthesized silver nanoparticles (Ag-NPs) have demonstrated promising effects, including cytotoxicity and anticancer potential, in different cell lines. Therefore, in our previous study, Ag-NPs were synthesized from the reduction of AgNO3 using Brassica rapa var. japonica (Bj) leaf extract as a reducing and stabilizing agent. The synthesized Ag-NPs were spherical in shape, with a size range of 15–30 nm. They had phase-centered cubic structure with strong growth inhibition potential against some bacteria. In continuation with our previous study, in the present study, we aimed to investigate the autophagy-regulated cytotoxic effect of Ag-NPs against human epithelial colorectal adenocarcinoma cells (Caco-2 cells). We found that the Bj leaf aqueous extract facilitated Brassica silver nanoparticles (Brassica Ag-NPs)-induced NF-κB mediated autophagy in Caco-2 cells. Results showed that Ag-NPs reduced cell viability of Caco-2 cells by inducing oxidative stress and DNA damage. Therefore, to understand the mechanism underlying the death-promoting activity of Ag-NPs in Caco-2 cells, western blotting was performed. Western blot analysis showed decreased expression of NFκB and increased expression of IκB, which is a sign of autophagy initiation. In addition, autophagosome formation was accelerated by the activity of p53 and light chain 3 (LC3) II. In addition, inhibition of Akt and mTOR also played a pivotal role in autophagy formation. Finally, excessive expansion of autophagy promoted apoptosis, which subsequently resulted in necrosis. These findings support a novel cell death-promoting function of autophagy by Ag-NPs in Caco-2 cells.
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页码:3272 / 3286
页数:14
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