Purification and bioactivity of exendin-4, a peptide analogue of GLP-1, expressed in Pichia pastoris

被引：0
|
作者：
Jin Zhou
Ju Chu
Yong-Hong Wang
Hui Wang
Ying-Ping Zhuang
Si-Liang Zhang
机构：
[1] East China University of Science & Technology,State Key Laboratory of Bioreactor Engineering, National Engineering Research Center for Biotechnology (Shanghai)
来源：
Biotechnology Letters | 2008年 / 30卷
关键词：
Diabetes; Exendin-4; Glucagon peptide analogue;
D O I：
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学科分类号：
摘要：
Exendin-4, a peptide analogue of glucagon-like peptide-1 (GLP-1), has been developed for treatment of type 2 diabetes. Herein, the secretive exendin-4 fusion protein, expressed by methanol induction in Pichia pastoris system, was purified to homogeneity by chromatography followed by enterokinase cleavage of the fusion protein and subsequent purification of the recombinant exendin-4. Purity of the recombinant exendin-4 was 95.6%. Bioactivity assay revealed that it had glucose-lowering and insulin-releasing action in vivo.
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页码：651 / 656
页数：5
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