eSGA: E. coli synthetic genetic array analysis

被引：0

作者：

Butland G. ^{[1
,2
,7
]}

Babu M. ^{[1
]}

Díaz-Mejía J.J. ^{[1
,3
]}

Bohdana F. ^{[1
]}

Phanse S. ^{[1
]}

Gold B. ^{[2
,7
]}

Yang W. ^{[2
,7
]}

Li J. ^{[1
]}

Gagarinova A.G. ^{[4
]}

Pogoutse O. ^{[1
]}

Mori H. ^{[5
]}

Wanner B.L. ^{[5
]}

Lo H. ^{[1
]}

Wasniewski J. ^{[1
]}

Christopoulos C. ^{[1
]}

Ali M. ^{[4
]}

Venn P. ^{[1
]}

Safavi-Naini A. ^{[1
]}

Sourour N. ^{[1
]}

Caron S. ^{[1
]}

Choi J.-Y. ^{[1
]}

Laigle L. ^{[1
]}

Nazarians-Armavil A. ^{[1
]}

Deshpande A. ^{[1
]}

Joe S. ^{[1
]}

Datsenko K.A. ^{[6
]}

Yamamoto N. ^{[5
]}

Andrews B.J. ^{[1
,4
]}

Boone C. ^{[1
,5
]}

Ding H. ^{[1
]}

Sheikh B. ^{[1
]}

Moreno-Hagelsieb G. ^{[3
]}

Greenblatt J.F. ^{[1
,4
]}

Emili A. ^{[1
,4
]}

机构：

[1] Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1

[2] Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720

[3] Department of Biology, Wilfrid Laurier University, Waterloo, ON N2L 3C5

[4] Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8

[5] Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630-0101

[6] Department of Biological Sciences, Purdue University, West Lafayette, IN 47907

[7] Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720

来源：

Nature Methods | 2008年 / 5卷 / 9期

基金：

日本科学技术振兴机构; 美国国家卫生研究院; 加拿大健康研究院;

关键词：

D O I：

10.1038/nmeth.1239

中图分类号：

学科分类号：

摘要：

Physical and functional interactions define the molecular organization of the cell. Genetic interactions, or epistasis, tend to occur between gene products involved in parallel pathways or interlinked biological processes. High-throughput experimental systems to examine genetic interactions on a genome-wide scale have been devised for Saccharomyces cerevisiae, Schizosaccharomyces pombe, Caenorhabditis elegans and Drosophila melanogaster, but have not been reported previously for prokaryotes. Here we describe the development of a quantitative screening procedure for monitoring bacterial genetic interactions based on conjugation of Escherichia coli deletion or hypomorphic strains to create double mutants on a genome-wide scale. The patterns of synthetic sickness and synthetic lethality (aggravating genetic interactions) we observed for certain double mutant combinations provided information about functional relationships and redundancy between pathways and enabled us to group bacterial gene products into functional modules.

引用

页码：789 / 795

页数：6

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