Enhanced conversion of sucrose to isomaltulose by a mutant of Erwinia rhapontici

被引:0
|
作者
Seung-Joon Ahn
Ji-Hyun Yoo
Hyeon-Cheol Lee
Sang-Yong Kim
Bong-Soo Noh
Jung-Hoe Kim
Jung-Kul Lee
机构
[1] BioNgene Co.,Department of Food & Microbial Technology
[2] Ltd,Department of Biological Sciences
[3] Seoul Women's University,undefined
[4] Korea Advanced Institute of Science and Technology,undefined
来源
Biotechnology Letters | 2003年 / 25卷
关键词
Bioscreen C; high-throughput screening; immobilization; isomaltulose;
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中图分类号
学科分类号
摘要
Mutagenesis of Erwinia rhapontici was performed to enhance the production of isomaltulose from sucrose. A mutant strain, BN 68089, was obtained through a screening process involving automated and miniaturized cultivation in Bioscreen C. This high-throughput, miniaturized screening system was optimized to identify the mutant strain, which had a conversion yield (90%) and productivity (194 g l−1 h−1). The BN 68089 mutant cells were immobilized in sodium alginate and when operated in a packed bed reactor gave a yield of 89% and a productivity of 144 g l−1 h−1 of at 30 °C, the optimal temperature. Immobilized BN 68089 cells exhibited 8% and 15% higher yield and productivity, respectively, than those of the wild-type strain.
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页码:1179 / 1183
页数:4
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