Extraction and quantification of phenolic acids and flavonols from Eugenia pyriformis using different solvents

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作者
Charles Windson Isidoro Haminiuk
Manuel Salvador Vicente Plata-Oviedo
Gisely de Mattos
Solange Teresinha Carpes
Ivanise Guilherme Branco
机构
[1] Universidade Tecnológica Federal do Paraná (UTFPR),Programa de Pós
[2] Universidade Tecnológica Federal do Paraná (UTFPR),Graduação em Tecnologia de Alimentos (PPGTA)
[3] Universidade Estadual Paulista (UNESP),Programa de Pós
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Phenolic acids; Flavonols; Extraction; Solvents; HPLC;
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摘要
The recovery of phenolic compounds of Eugenia pyriformis using different solvents was investigated in this study. The compounds were identified and quantified by reverse-phase high-performance liquid chromatography coupled with ultraviolet–visible diode-array detector (RP-HPLC-DAD/UV–vis). Absolute methanol was the most effective extraction agent of phenolic acids and flavonols (588.31 mg/Kg) from Eugenia pyriformis, although similar results (p ≤ 0.05) were observed using methanol/water (1:1 ratio). Our results clearly showed that higher contents of phenolic compounds were not obtained either with the most or the least polar solvents used. Several phenolic compounds were identified in the samples whereas gallic acid and quercetin were the major compounds recovered.
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页码:2862 / 2866
页数:4
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