In situ proteolysis for protein crystallization and structure determination

被引：0
|
作者：
Aiping Dong
Xiaohui Xu
Aled M Edwards
机构：
[1] Structural Genomics Consortium,
[2] University of Toronto,undefined
[3] Midwest Center for Structural Genomics,undefined
[4] University of Toronto,undefined
来源：
Nature Methods | 2007年 / 4卷
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学科分类号：
摘要：
We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.
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页码：1019 / 1021
页数：2
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