Transformation of the phytopathogen Mycosphaerella graminicola to carbendazim and hygromycin B resistance

被引：0
|
作者：
A. C. Payne
Marie-Claire Grosjean-Cournoyer
Derek W. Hollomon
机构：
[1] IACR-Long Ashton Research Station,
[2] Department of Agricultural Sciences,undefined
[3] University of Bristol,undefined
[4] Long Ashton,undefined
[5] Bristol,undefined
[6] BS41 9AF,undefined
[7] UK e-mail: Andrew.Payne@BBSRC.AC.UK Fax: +33-4-1275 394007,undefined
[8] Rhone-Poulenc AGRO,undefined
[9] 14 rue Pierre Baizet,undefined
[10] B.P. 9163,undefined
[11] F-69263 Lyon Cedex 09,undefined
[12] France,undefined
来源：
Current Genetics | 1998年 / 34卷
关键词：
Key wordsSeptoria tritici; β-Tubulin;
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学科分类号：
摘要：
The fungal wheat pathogen Mycosphaerella graminicola was transformed to carbendazim and hygromycin B resistance. A β-tubulin gene from a M. graminicola strain resistant to the fungicide carbendazim was cloned and used to transform a sensitive strain to carbendazim resistance. Hygromycin B-resistant transformants (up to 8 per μg of transforming DNA) arose at a higher rate than β-tubulin transformants (up to 0.6 per μg of DNA). Transformants were able to infect wheat and were morphologically similar to recipient strains.
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页码：100 / 104
页数：4
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