A Discussion of Molecular Biology Methods for Protein Engineering

被引：0
|
作者：
Alexander Zawaira
Anil Pooran
Samantha Barichievy
Denis Chopera
机构：
[1] Synthetic Biology–ERA,Gene Expression and Biophysics Group
[2] University of Cape Town Lung Institute,Department of Medicine, Lung Infection and Immunity Unit, Division of Pulmonology
[3] Observatory,Division of Medical Virology, Institute for Infectious Disease and Molecular Medicine, Faculty of Health Sciences
[4] University of Cape Town,undefined
[5] Observatory,undefined
来源：
Molecular Biotechnology | 2012年 / 51卷
关键词：
Molecular biology; Protein engineering; Mutagenesis; Site-directed mutagenesis; Insertion mutagenesis; Deletion mutagenesis; Polymerase chain reaction; Molecular cloning; Recombinant protein expression; Recombinant plasmid;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A number of molecular biology techniques are available to generate variants from a particular start gene for eventual protein expression. We discuss the basic principles of these methods in a repertoire that may be used to achieve the elemental steps in protein engineering. These include site-directed, deletion and insertion mutagenesis. We provide detailed case studies, drawn from our own experiences, packaged together with conceptual discussions and include an analysis of the techniques presented with regards to their uses in protein engineering.
引用
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页码：67 / 102
页数：35
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