Ultrastructural localization of the NADPH-diaphorase activity in the Leydig cells of aging mice

被引：0
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作者：
R. Ruffoli
Maria Anita Giambelluca
Franco Giannessi
Paola Soldani
Lucia Grasso
Maurizio Gasperi
Francesco Giannessi
机构：
[1] Dipartimento di Morfologia Umana e Biologia Applicata,
[2] Facoltà di Medicina e Chirurgia,undefined
[3] Università degli Studi di Pisa,undefined
[4] Via Roma,undefined
[5] 55–56126 Pisa,undefined
[6] Italy e-mail: rruffoli@anist.med.unipi.it Tel.: +39-050-835911,undefined
[7] Fax: +39-050-835926,undefined
[8] Dipartimento di Matematica,undefined
[9] Facoltà di Scienze Matematiche,undefined
[10] Fisiche e Naturali,undefined
[11] Università degli Studi di Pisa,undefined
[12] Via Buonarroti 2,undefined
[13] 56100 Pisa,undefined
[14] Italy,undefined
[15] Dipartimento di Endocrinologia e Metabolismo,undefined
[16] Ortopedia e Traumatologia,undefined
[17] Medicina del Lavoro,undefined
[18] Facoltà di Medicina e Chirurgia,undefined
[19] Università degli Studi di Pisa,undefined
[20] Via Paradisa 2,undefined
[21] 56100 Pisa,undefined
[22] Italy,undefined
来源：
Anatomy and Embryology | 2001年 / 203卷
关键词：
Keywords Leydig cell; Ultrastructure; Aging; Mouse; Nitric oxide;
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摘要：
Recently, it has been shown that nitric oxide may inhibit the Leydig cell steroidogenesis. The present paper describes, by means of NADPH-diaphorase histochemistry, the ultrastructural localization of the enzyme nitric oxide synthase in the Leydig cells of young adult and aging mice. In the young adult mice, the enzymatic reaction was mainly located in the mitochondria and in some clustered cisternæ of the smooth endoplasmic reticulum. The nuclear envelope was faintly labeled. In the aging mice, most Leydig cells showed an enhanced enzymatic reaction. Labeled mitochondria were increased in number, and labeled areas of the smooth endoplasmic reticulum were more numerous and extended. In addition, a strong enzymatic reaction was recognized in the nuclear envelope. We conjecture that the impaired steroidogenesis observed in the testis of aging mammals might, at least in part, depend on the increased nitric oxide production in the Leydig cells.
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页码：383 / 391
页数：8
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