In this study, a single-cylinder direct-injection spark-ignition research engine with full optical access was used to investigate the influence of the flow field and fuel/air mixing on cyclic variability, in particular in the early flame propagation. The engine was operated under lean-burn conditions at 1500 rpm. Two different injection strategies were compared, port-fuel injection (PFI) and direct injection (DI), the latter with early and late injection split about 2:1 in fuel mass. High-speed particle image velocimetry captured the flow in the tumble plane in the compression stroke. The velocity fields and the movement of the tumble vortex are analyzed. Simultaneously, a second camera detected the chemiluminescence of the flame, and the projected area of the line-of-sight-integrated flame luminosity was extracted through morphological image processing. By combining pressure-based combustion analysis and high-speed optical diagnostics, the early flame propagation and the flow field are correlated. In separate experiments the equivalence ratio was imaged for the DI at selected crank angles and correlated with CA10 to learn about the influence of mixture inhomogeneity on early flame propagation. With PFI, the flow near the spark plug just before ignition is closely related to the subsequent speed of combustion. The combustion-relevant flow features can be traced back in time to about –90 °CA. In contrast, the chosen DI scheme results in a decorrelation of flow and flame, and the equivalence ratio distribution at ignition becomes more important. For both flow and mixture fields, regions of high correlation with early-combustion metrics are typically associated with gradients in the multi-cycle average fields.