Cold sensitivity of the SARS-CoV-2 spike ectodomain

被引：0

作者：

Robert J. Edwards

Katayoun Mansouri

Victoria Stalls

Kartik Manne

Brian Watts

Rob Parks

Katarzyna Janowska

Sophie M. C. Gobeil

Megan Kopp

Dapeng Li

Xiaozhi Lu

Zekun Mu

Margaret Deyton

Thomas H. Oguin

Jordan Sprenz

Wilton Williams

Kevin O. Saunders

David Montefiori

Gregory D. Sempowski

Rory Henderson

S. Munir Alam

Barton F. Haynes

Priyamvada Acharya

机构：

[1] Duke Human Vaccine Institute,Department of Medicine

[2] Duke University,Department of Surgery

[3] Duke University,Department of Immunology

[4] Duke University,undefined

来源：

Nature Structural & Molecular Biology | 2021年 / 28卷

关键词：

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学科分类号：

摘要：

The SARS-CoV-2 spike (S) protein, a primary target for COVID-19 vaccine development, presents its receptor binding domain in two conformations, the receptor-accessible ‘up’ or receptor-inaccessible ‘down’ states. Here we report that the commonly used stabilized S ectodomain construct ‘2P’ is sensitive to cold temperatures, and this cold sensitivity is abrogated in a ‘down’ state-stabilized ectodomain. Our findings will impact structural, functional and vaccine studies that use the SARS-CoV-2 S ectodomain.

引用

页码：128 / 131

页数：3

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