Deubiquitinase-based analysis of ubiquitin chain architecture using Ubiquitin Chain Restriction (UbiCRest)

被引：0
|
作者：
Manuela K Hospenthal
Tycho E T Mevissen
David Komander
机构：
[1] Medical Research Council,
[2] Laboratory of Molecular Biology,undefined
[3] Present address: Institute of Structural and Molecular Biology,undefined
[4] Birkbeck College,undefined
[5] London,undefined
[6] UK.,undefined
来源：
Nature Protocols | 2015年 / 10卷
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学科分类号：
摘要：
UbiCRest is a simple PAGE-based method that leverages the varying specificities of deubiquitinating enzymes (DUBs) to generate information about the composition and architecture of ubiquitin chains.
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页码：349 / 361
页数：12
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