In vitro induction of multiple shoots and plant regeneration in Vigna

Cotyledonary nodes, excised cotyledons, and hypocotyl segments of six varieties of Vigna mungo and V. radiata have been tested for their morphogenic potential on media containing a range of hormonal combinations including benzyladenine, kinetin, thidiazuron (TDZ), and zeatin. Multiple shoots developed on cotyledonary node explants in all varieties tested on basal medium containing cytokinin. Presence of both the cotyledons, either full or half, resulted in a maximum number of shoots produced. Shoot bud regeneration was achieved via meristem formation on excised cotyledons on Murashige-Skoog basal medium with B5 vitamins supplemented with TDZ. Mature plants had normal phenotypes. V. mungo var. PS1 and V. radiata var. Pusa105 were found to be the most responsive varieties for shoot regeneration. The histology of in vitro organogenesis was studied.