Strategies for signal amplification in nucleic acid detection

被引：0

作者：

S. Calin Andras

J. Brian Power

Edward C. Cocking

Michael R. Davey

机构：

[1] University of Nottingham,Plant Science Division, School of Biosciences

[2] “Babes-Bolyai” University,Faculty of Biology and Geology, Ecology and Genetics Department

来源：

Molecular Biotechnology | 2001年 / 19卷

关键词：

Signal amplification; nucleic acid detection; hybridization; PCR; primed ; labeling; Self-Sustained Sequence Replication; Strand Displacement Amplification; Ligase Chain Reaction; Padlock Probes; Rolling Circle Amplification; Tyramide Signal Amplification; Branched DNA Amplification;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Many aspects of molecular genetics necessitate the detection of nucleic acid sequences. Current approaches involving target amplification (in situ PCR, Primed in situ Labeling, Self-Sustained Sequence Replication, Strand Displacement Amplification), probe amplification (Ligase Chain Reaction, Padlock Probes, Rolling Circle Amplification) and signal amplification (Tyramide Signal Amplification, Branched DNA Amplification) are summarized in the present review, together with their advantages and limitations.

引用

页码：29 / 44

页数：15

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