Serodiagnosis, targeting nonstructural protein 4, of porcine reproductive and respiratory syndrome virus

被引:0
|
作者
Shengliang Cao
Xinna Cai
Min Tan
Fachao Sun
Yuchao Wang
Yingli Shang
Sidang Liu
Yihong Xiao
机构
[1] Shandong Agricultural University,Department of Fundamental Veterinary Medicine, College of Animal Science and Veterinary Medicine
[2] Shandong Agricultural University,Department of Preventive Veterinary Medicine, College of Animal Science and Veterinary Medicine
[3] Shandong Agricultural University,Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention
来源
Archives of Virology | 2018年 / 163卷
关键词
D O I
暂无
中图分类号
学科分类号
摘要
Porcine reproductive and respiratory syndrome virus (PRRSV) causes great economic losses to the swine industry worldwide. Typically, an N protein-coated indirect enzyme-linked immunosorbent assay (N-coated iELISA) is used to detect PRRSV antibodies. Non-structural protein (NSP) 4 is essential to the PRRSV life cycle and contains B-cell epitopes. Yet, no specific antibody against NSP4 has been detected in clinical samples. In this study, we developed an NSP4-coated iELISA and compared its effectiveness with the N-coated iELISA. The NSP4-coated iELISA was developed with a cut-off value of 0.406 at an optical density of 450 nm by testing a panel of 70 PRRSV positive and 80 PRRSV negative pig serum samples, which generated a specificity and sensitivity of 100%. Agreement between the NSP4-coated and N-coated iELISAs was 92.2%. Interestingly, 50 serum samples, mostly from pigs vaccinated with the HP-PRRSV live strain, tested positive for PRRSV antibodies with the NSP4-coated iELISA, but were negative with the N-coated iELISA. These results were further confirmed by western blot analysis and another iELISA based on the N-terminus of NSP2 (NSP2-1-coated iELISA). The agreement between the results of western blot analysis with the NSP4-coated and NSP2-1-coated iELISA analyses were 92% and 96.1%, respectively, showing that the developed NSP4-coated iELISA is a useful tool to discriminate a false negative from a true negative response to the HP-PRRSV vaccine.
引用
收藏
页码:411 / 418
页数:7
相关论文
共 50 条
  • [1] Serodiagnosis, targeting nonstructural protein 4, of porcine reproductive and respiratory syndrome virus
    Cao, Shengliang
    Cai, Xinna
    Tan, Min
    Sun, Fachao
    Wang, Yuchao
    Shang, Yingli
    Liu, Sidang
    Xiao, Yihong
    ARCHIVES OF VIROLOGY, 2018, 163 (02) : 411 - 418
  • [2] Induction of Apoptosis by the Nonstructural Protein 4 and 10 of Porcine Reproductive and Respiratory Syndrome Virus
    Yuan, Shuaizhen
    Zhang, Ning
    Xu, Lei
    Zhou, Lei
    Ge, Xinna
    Guo, Xin
    Yang, Hanchun
    PLOS ONE, 2016, 11 (06):
  • [3] A Nanobody Targeting Viral Nonstructural Protein 9 Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication
    Wang, Lizhen
    Zhang, Lu
    Huang, Baichen
    Li, Kuokuo
    Hou, Gaopeng
    Zhao, Qin
    Wu, Chunyan
    Nan, Yuchen
    Du, Taofeng
    Mu, Yang
    Lan, Jixun
    Chen, Hongying
    Zhou, En-Min
    JOURNAL OF VIROLOGY, 2019, 93 (04)
  • [4] Mapping the Nonstructural Protein Interaction Network of Porcine Reproductive and Respiratory Syndrome Virus
    Song, Jiangwei
    Liu, Yuanyuan
    Gao, Peng
    Hu, Yunhao
    Chai, Yue
    Zhou, Shaochuan
    Kong, Can
    Zhou, Lei
    Ge, Xinna
    Guo, Xin
    Han, Jun
    Yang, Hanchun
    JOURNAL OF VIROLOGY, 2018, 92 (24)
  • [5] Mutagenesis analysis of porcine reproductive and respiratory syndrome virus nonstructural protein 7
    Minze Zhang
    Zhenpeng Cao
    Jiexiong Xie
    Wanjun Zhu
    Pei Zhou
    Honglang Gu
    Long Sun
    Shuo Su
    Guihong Zhang
    Virus Genes, 2013, 47 : 467 - 477
  • [6] Mutagenesis analysis of porcine reproductive and respiratory syndrome virus nonstructural protein 7
    Zhang, Minze
    Cao, Zhenpeng
    Xie, Jiexiong
    Zhu, Wanjun
    Zhou, Pei
    Gu, Honglang
    Sun, Long
    Su, Shuo
    Zhang, Guihong
    VIRUS GENES, 2013, 47 (03) : 467 - 477
  • [7] Porcine Reproductive and Respiratory Syndrome Virus Nonstructural Protein 4 Antagonizes Beta Interferon Expression by Targeting the NF-κB Essential Modulator
    Huang, Chen
    Zhang, Qiong
    Guo, Xue-kun
    Yu, Zhi-bin
    Xu, Ao-Tian
    Tang, Jun
    Feng, Wen-hai
    JOURNAL OF VIROLOGY, 2014, 88 (18) : 10934 - 10945
  • [8] Suppression of immune responses in pigs by nonstructural protein 1 of Porcine reproductive and respiratory syndrome virus
    Zhou, Yefei
    Bai, Juan
    Li, Yufeng
    Wang, Xinglong
    Wang, Xianwei
    Jiang, Ping
    CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE, 2012, 76 (04): : 255 - 260
  • [9] Porcine Reproductive and Respiratory Syndrome Virus Nonstructural Protein 4 Cleaves Porcine DCP1a To Attenuate Its Antiviral Activity
    Tao, Ran
    Fang, Liurong
    Bai, Dongcheng
    Ke, Wenting
    Zhou, Yanrong
    Wang, Dang
    Xiao, Shaobo
    JOURNAL OF IMMUNOLOGY, 2018, 201 (08): : 2345 - 2353
  • [10] Porcine reproductive and respiratory syndrome virus nonstructural protein 2 contributes to NF-κB activation
    Fang, Ying
    Fang, Liurong
    Wang, Yang
    Lei, Yingying
    Luo, Rui
    Wang, Dang
    Chen, Huanchun
    Xiao, Shaobo
    VIROLOGY JOURNAL, 2012, 9