Improvement of growth and camptothecin yield by altering nitrogen source supply in cell suspension cultures of Camptotheca acuminata

被引：0
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作者：
Xue-wu Pan
Heng-hao Xu
Xin liu
Xiang Gao
Ying-tang lu
机构：
[1] College of Life Sciences,Key Lab of MOE for Plant Developmental Biology
[2] Wuhan University,undefined
来源：
Biotechnology Letters | 2004年 / 26卷
关键词：
ammonium; camptothecin; nitrate; two-stage culture;
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摘要：
Nitrate at 70 m gave the highest biomass of Camptotheca acuminata in suspension culture in MS medium, but a NH4+/NO3− molar ratio of 5:1 (giving a total of 40 m N) gave the maximum camptothecin yield. A two-stage flask culture system was established to improve culture efficiency; cell dry weight, camptothecin content and yield was increased by 30%, 280% and 340%, respectively when compared with those of control, reaching up to 36 g l−1, 0.36 mg g−1, and 12.8 mg l−1, respectively.
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页码：1745 / 1748
页数：3
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