Regeneration of garlic callus as affected by clonal variation, plant growth regulators and culture conditions over time

被引：0
|
作者：
J. M. Myers
P. W. Simon
机构：
[1] USDA-ARS Vegetable Crops Research,
[2] Department of Horticulture,undefined
[3] 1575 Linden Drive,undefined
[4] University of Wisconsin-Madison,undefined
[5] Madison,undefined
[6] WI 53706,undefined
[7] USA e-mail: psimon@facstaff.wisc.edu Fax: +608-262-4743,undefined
来源：
Plant Cell Reports | 1999年 / 19卷
关键词：
Key words Allium sativum; Benzyladenine; Picloram; Thidiazuron; 2; 4-D; 2iP;
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摘要：
 A long-term regeneration system for garlic (Allium sativum L.) clones of diverse origin was developed. Callus was initiated on a modified Gamborg's B-5 medium supplemented with 4.5 μM 2,4-D and maintained on the same basal medium with 4.7 μM picloram+0.49 μM 2iP. Regeneration potential of callus after 5, 12 and 16 months on maintenance medium was measured using several plant growth regulator treatments. The 1.4 μM picloram+13.3 μM BA treatment stimulated the highest rate of shoot production. Regeneration rate decreased as callus age increased, but healthy plantlets from callus cultures up to 16-months-old were produced for all clones. Regeneration of long-term garlic callus cultures could be useful for clonal propagation and transformation.
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页码：32 / 36
页数：4
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