Isolation of myo-Inositol from Solutions by Enzymatic Biotransformation

被引：0
|
作者：
E. N. Reut
M. M. Rakhimov
机构：
[1] Mirzo Ulugbek National University of Uzbekistan,
来源：
Applied Biochemistry and Microbiology | 2003年 / 39卷
关键词：
Alcohol; Methanol; Hexane; Optimum Condition; Organic Solvent;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The transferase reaction between phospholipids and inositol catalyzed by phospholipase D was studied at interfaces in water–organic solvent systems. Optimum conditions were determined for phosphatidylinositol synthesis in heterogeneous water–organic solvent systems. Hydrophobic components (phospholipids) were readily separated from water-soluble products (alcohols) in systems with organic solvents. In the hexane–water system, addition of methanol (an alcohol substrate) to the reaction medium displaced myo-inositol from the molecule of phosphatidylinositol. myo-Inositol was isolated from the mixture of its isomers using a two-step transferase reaction catalyzed by phospholipase D.
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页码：363 / 367
页数：4
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