Streptomyces camelliae sp. nov., isolated from rhizosphere soil of Camellia oleifera Abel

A novel actinobacterium strain, designated HUAS 2-6( T), was isolated from the rhizosphere soil of Camellia oleifera Abel collected from Taoyuan County, Northwestern Hunan Province, South China. This strain was subjected to a polyphasic taxonomic study. Strain HUAS 2-6( T) is characterized by morphology typical of members of the genus Streptomyces, with deep purplish vinaceous aerial mycelia and deep dull lavender substrate mycelia. Strain HUAS 2-6( T), based on the full-length 16S rRNA gene sequence analysis, exhibited the highest similarities to S. puniciscabiei S77(T) (99.31%), S. filipinensis NBRC 12860( T) (99.10%), S. yaanensis CGMCC 4.7035( T) (99.09%), S. fodineus TW1S1(T) (99.08%), S. broussonetiae CICC 24819( T) (98.76%), S. achromogenes JCM 4121( T) (98.69%), S. barringtoniae JA03(T) (98.69%), and less than 98.70% with other validly species. In phylogenomic tree, strain HUAS 2-6( T) was clustered together with S. broussonetiae CICC 24819( T), suggesting that they were closely related to each other. However, average nucleotide identity (ANI) and digital DNA-DNA hybridisation (dDDH) between them were much less than the species cutoff values (ANI 96.7% and dDDH 70%). Moreover, in phenotypic and chemotaxonomic characteristics, strain HUAS 2-6( T) is distinct from S. broussonetiae CICC 24819( T). On the basis of the polyphasic data, strain HUAS 2-6( T) is proposed to represent a novel species, Streptomyces camelliae sp. nov. (= MCCC 1K04729(T) = JCM 35918( T)).