Expression and purification of an engineered human endothelin receptor B in a monomeric form

被引：0
|
作者：
A. V. Mishin
A. P. Luginina
A. P. Potapenko
V. I. Borshchevskiy
V. Katritch
E. Edelweiss
I. S. Okhrimenko
V. I. Gordeliy
V. G. Cherezov
机构：
[1] Moscow Institute of Physics and Technology (State University),Bridge Institute, Department of Chemistry
[2] University of Southern California,CNRS
[3] The European Molecular Biology Laboratory (EMBL),Universite Grenoble Alpes
[4] IBS,CEA
[5] IBS,Institute of Complex Systems (ICS), ICS
[6] IBS,6: Molecular Biophysics
[7] Research Centre Juelich,undefined
来源：
Doklady Biochemistry and Biophysics | 2016年 / 467卷
关键词：
DOKLADY Biochemistry; Detergent Micelle; Denaturation Curve; Detergent Mixture; GPCR Receptor;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
In humans, two endothelin receptors, ETa and ETb, are activated by three endogenous 21-mer cyclic peptides, ET-1, ET-2, and ET-3, which control various physiological processes, including vasoconstriction, vasodilation, and stimulation of cell proliferation. The first stage of this study it to produce a stable solubilized and purified receptor in a monodisperse state. This article is focused on the engineering, expression, purification, and characterization of the endothelin receptor B for subsequent structural and functional studies.
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页码：157 / 161
页数：4
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