Optimization of pulsed field gel electrophoresis (PFGE) conditions for thermophilic bacilli

被引：0
|
作者：
Elif Yavuz
Hatice Gunes
Sebnem Harsa
Cisem Bulut
A. Fazil yenidunya
机构：
[1] Izmir Institute of Technology,Biotechnology and Bioengineering Programme
[2] Izmir Institute of Technology,Department of Biology, Science Faculty
来源：
World Journal of Microbiology and Biotechnology | 2004年 / 20卷
关键词：
DNA degradation; genotyping; pulsed field gel electrophoresis; thermophilic ;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Although thermophilic members of the genus Bacillus are known to tolerate extreme environmental conditions, they appeared to be readily damaged upon mechanical manipulations. This fact was evidenced in genotyping of some strains of thermophilic Bacillus by pulsed field gel electrophoresis (PFGE). Consequently, a new procedure for the preparation of agarose plugs was developed. The procedure produced interpretable genomic DNA restriction profiles.
引用
收藏
页码：871 / 874
页数：3
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