Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software

被引：0

作者：

Daniel Sage

Thanh-An Pham

Hazen Babcock

Tomas Lukes

Thomas Pengo

Jerry Chao

Ramraj Velmurugan

Alex Herbert

Anurag Agrawal

Silvia Colabrese

Ann Wheeler

Anna Archetti

Bernd Rieger

Raimund Ober

Guy M. Hagen

Jean-Baptiste Sibarita

Jonas Ries

Ricardo Henriques

Michael Unser

Seamus Holden

机构：

[1] Ecole Polytechnique Fédérale de Lausanne,Biomedical Imaging Group, School of Engineering

[2] Harvard University,Harvard Center for Advanced Imaging

[3] STI - IBI,Laboratory of Nanoscale Biology and Laboratoire d’Optique Biomédicale

[4] Ecole Polytechnique Fédérale de Lausanne,Department of Radioelectronics

[5] FEE,University of Minnesota Informatics Institute

[6] Czech Technical University,Department of Biomedical Engineering

[7] University of Minnesota Twin Cities,Department of Molecular and Cellular Medicine

[8] Texas A&M University,Department of Microbial Pathogenesis and Immunology

[9] Texas A&M University Health Science Center,MRC Genome Damage and Stability Centre, School of Life Sciences

[10] Texas A&M University Health Science Center,Advanced Imaging Resource, Institute of Genetics and Molecular Medicine

[11] University of Sussex,Laboratory of Experimental Biophysics

[12] Double Helix LLC,Department of Imaging Physics

[13] Istituto Italiano di Tecnologia,Centre for Cancer Immunology

[14] University of Edinburgh,UCCS Center for the Biofrontiers Institute

[15] École Polytechnique Fédérale de Lausanne,Interdisciplinary Institute for Neuroscience

[16] Delft University of Technology,Interdisciplinary Institute for Neuroscience

[17] University of Southampton,European Molecular Biology Laboratory

[18] University of Colorado,Quantitative Imaging and Nanobiophysics Group, MRC Laboratory for Molecular Cell Biology

[19] University of Bordeaux,Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences

[20] Centre National de la Recherche Scientifique (CNRS) UMR 5297,undefined

[21] Cell Biology and Biophysics Unit,undefined

[22] University College London,undefined

[23] Newcastle University,undefined

来源：

Nature Methods | 2019年 / 16卷

关键词：

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中图分类号：

学科分类号：

摘要：

With the widespread uptake of two-dimensional (2D) and three-dimensional (3D) single-molecule localization microscopy (SMLM), a large set of different data analysis packages have been developed to generate super-resolution images. In a large community effort, we designed a competition to extensively characterize and rank the performance of 2D and 3D SMLM software packages. We generated realistic simulated datasets for popular imaging modalities—2D, astigmatic 3D, biplane 3D and double-helix 3D—and evaluated 36 participant packages against these data. This provides the first broad assessment of 3D SMLM software and provides a holistic view of how the latest 2D and 3D SMLM packages perform in realistic conditions. This resource allows researchers to identify optimal analytical software for their experiments, allows 3D SMLM software developers to benchmark new software against the current state of the art, and provides insight into the current limits of the field.

引用

页码：387 / 395

页数：8

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