Blotting protein complexes from native gels to electron microscopy grids

被引：0
|
作者：
Roland Wilhelm Knispel
Christine Kofler
Marius Boicu
Wolfgang Baumeister
Stephan Nickell
机构：
[1] Max Planck Institute of Biochemistry,Department of Molecular Structural Biology
[2] Present addresses: ChemAxon Kft.,undefined
[3] Budapest,undefined
[4] Hungary (R.W.K.),undefined
[5] Tietz Video and Image Processing Systems GmbH,undefined
[6] Gauting,undefined
[7] Germany (C.K.) and Carl Zeiss NTS GmbH,undefined
[8] Oberkochen,undefined
[9] Germany (S.N.).,undefined
来源：
Nature Methods | 2012年 / 9卷 / 2期
关键词：
D O I：
10.1038/nmeth.1840
中图分类号：
学科分类号：
摘要：
A simple, general procedure for transferring protein complexes directly from native gels to electron microscopy grids for structural analysis is reported.
引用
收藏
页码：182 / 184
页数：2
相关论文
共 50 条
  • [41] Methodical aspects of the electron microscopy of biopolymer gels
    I. I. Chemeris
    T. G. Kalinichenko
    A. G. Filatova
    E. M. Belavtseva
    Bulletin of the Russian Academy of Sciences: Physics, 2007, 71 (10) : 1458 - 1460
  • [42] ELECTRON MICROSCOPY OF CARRAGEENAN-MILK GELS
    HOOD, LF
    JOURNAL OF DAIRY SCIENCE, 1971, 54 (05) : 767 - &
  • [43] Electron microscopy of biotinylated protein complexes bound to streptavidin monolayer crystals
    Han, Bong-Gyoon
    Walton, Ross W.
    Song, Amos
    Hwu, Peter
    Stubbs, Milton T.
    Yannone, Steven M.
    Arbelaez, Pablo
    Dong, Ming
    Glaeser, Robert M.
    JOURNAL OF STRUCTURAL BIOLOGY, 2012, 180 (01) : 249 - 253
  • [44] Structural analysis of membrane protein complexes by single particle electron microscopy
    Rubinstein, John L.
    METHODS, 2007, 41 (04) : 409 - 416
  • [45] A SIMPLE DEVICE FOR STAINING ELECTRON-MICROSCOPY GRIDS
    HAKONSEN, MB
    GROTHE, AG
    JORGENSEN, L
    ULTRASTRUCTURAL PATHOLOGY, 1984, 7 (04) : 335 - 337
  • [46] PREPARING GRIDS FOR ELECTRON-MICROSCOPY OF FECAL SAMPLES
    ROBERTS, JL
    CURRY, A
    PAVER, WK
    LANCET, 1980, 2 (8186): : 151 - 152
  • [47] BLOTTING OF SEED PROTEINS FROM ISOELECTRICALLY FOCUSED GELS FOR CULTIVAR IDENTIFICATION
    MCDONALD, MB
    SEED SCIENCE AND TECHNOLOGY, 1991, 19 (01) : 33 - 40
  • [48] ISOLATION AND CHARACTERIZATION OF CHLOROPHYLL-PROTEIN COMPLEXES FROM POLYACRYLAMIDE GELS
    DUNAHAY, TG
    STAEHELIN, LA
    JOURNAL OF CELL BIOLOGY, 1984, 99 (04): : A17 - A17
  • [49] RECONSTITUTION OF PROTEIN COMPLEXES ISOLATED FROM SDS POLYACRYLAMIDE GELS INTO LIPOSOMES
    GOODMAN, TL
    STAEHELIN, LA
    JOURNAL OF CELL BIOLOGY, 1982, 95 (02): : A259 - A259
  • [50] Variability of Protein Structure Models from Electron Microscopy
    Monroe, Lyman
    Terashi, Genki
    Kihara, Daisuke
    STRUCTURE, 2017, 25 (04) : 592 - +
12345