Sunflower Nitrate Reductase: Purification and Characterization

被引：0
|
作者：
Debasis Pattanayak
Sukumar R. Chatterjee
机构：
[1] Indian Agricultural Research Institute,Division of Biochemistry
[2] Indian Agricultural Research Institute,Nuclear Research Laboratory
[3] Central Potato Research Institute,Division of Crop Physiology & Biochemistry
来源：
Journal of Plant Biochemistry and Biotechnology | 1999年 / 8卷
关键词：
nitrate reductase; sunflower; blue Sepharose CL-6B; molecular weight; sub-unit; pH optima; ferric citrate reductase;
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学科分类号：
摘要：
A single isoform, NADH: nitrate reductase (NR), was purified 500 folds from sunflower leaves by affinity chromatography on Blue Sepharose CL-6B. Purified NR had a pH optima of 7.25 and a molecular weight of 210 kD. In SDS-PAGE, two bands of 47 and 56 kD were obtained. NADH: ferric citrate reductase activity was copurified with NR with a specific activity of 2. The Vmax of NADH: ferric citrate reductase was 8.69 units mg-1 protein and the apparent Km for ferric citrate was 0.435 mM.
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页码：41 / 45
页数：4
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