Evaluation of combined high-efficiency DNA extraction and real-time PCR for detection of Mycobacterium avium subsp. paratuberculosis in subclinically infected dairy cattle: comparison with faecal culture, milk real-time PCR and milk ELISA

被引:0
|
作者
Katarina Logar
Rok Kopinč
Petra Bandelj
Jože Starič
Aleš Lapanje
Matjaž Ocepek
机构
[1] Institute of Microbiology and Parasitology,Veterinary Faculty
[2] Institute of Physical Biology,Veterinary Faculty
[3] Clinic for Ruminants,undefined
来源
关键词
Faecal Sample; Milk Sample; Body Condition Score; Internal Amplification Control; Faecal Culture;
D O I
暂无
中图分类号
学科分类号
摘要
引用
收藏
相关论文
共 50 条
  • [31] Sensitivity of solid culture, broth culture, and real-time PCR assays for milk and colostrum samples from Mycobacterium avium ssp paratuberculosis-infectious dairy cows
    Laurin, Emilie
    McKenna, Shawn
    Chaffer, Marcelo
    Keefe, Greg
    JOURNAL OF DAIRY SCIENCE, 2015, 98 (12) : 8597 - 8609
  • [32] Comparison between digital PCR and real-time PCR in detection of Salmonella typhimurium in milk
    Wang, Meng
    Yang, Junjie
    Gai, Zhongtao
    Huo, Shengnan
    Zhu, Jianhua
    Li, Jun
    Wang, Ranran
    Xing, Sheng
    Shi, Guosheng
    Shi, Feng
    Zhang, Lei
    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2018, 266 : 251 - 256
  • [33] Highly Specific and Quick Detection of Mycobacterium avium subsp paratuberculosis in Feces and Gut Tissue of Cattle and Humans by Multiple Real-Time PCR Assays
    Imirzalioglu, Can
    Dahmen, Heinrich
    Hain, Torsten
    Billion, Andre
    Kuenne, Carsten
    Chakraborty, Trinad
    Domann, Eugen
    JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (05) : 1843 - 1852
  • [34] Mycobacterium avium subsp paratuberculosis survival during fermentation of soured milk products detected by culture and quantitative real time PCR methods
    Klanicova, B.
    Slana, I.
    Roubal, P.
    Pavlik, I.
    Kralik, P.
    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2012, 157 (02) : 150 - 155
  • [35] Examination of Mycobacterium avium subsp. avium distribution in naturally infected hens by culture and triplex quantitative real time PCR
    Kaevska, M.
    Slana, I.
    Kralik, P.
    Pavlik, I.
    VETERINARNI MEDICINA, 2010, 55 (07) : 325 - 330
  • [36] Detection of Mycobacterium avium subsp paratuberculosis in bovine faecal samples by Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) TaqMan Assay
    Rasool, S.
    Narang, D.
    Chandra, M.
    Sharma, N. S.
    Singh, Tejinder S.
    TROPICAL BIOMEDICINE, 2016, 33 (02) : 327 - 334
  • [37] Optimization of methods for detecting Mycobacterium avium subsp paratuberculosis in environmental samples using quantitative, real-time PCR
    Cook, Kimberly L.
    Britt, Jenks S.
    JOURNAL OF MICROBIOLOGICAL METHODS, 2007, 69 (01) : 154 - 160
  • [38] New triplex real-time PCR assay for detection of Mycobactetium avium subsp paratuberculosis in bovine feces
    Schoenenbrucher, H.
    Abdurnawjood, A.
    Failing, K.
    Buelte, M.
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (09) : 2751 - 2758
  • [39] Establishment a real-time reverse transcription PCR based on host biomarkers for the detection of the subclinical cases of Mycobacterium avium subsp paratuberculosis
    Park, Hyun-Eui
    Park, Hong-Tae
    Jung, Young Hoon
    Yoo, Han Sang
    PLOS ONE, 2017, 12 (05):
  • [40] Evaluation of methods of DNA extraction from Staphylococcus aureus in milk for use in real-time PCR
    Dibbern, A. G.
    Botaro, B. G.
    Viziack, M. P.
    Silva, L. F. P.
    Santos, M. V.
    GENETICS AND MOLECULAR RESEARCH, 2015, 14 (01) : 227 - 233