Application of a quantitative 1H-NMR (1H-qNMR) method for the determination of geniposidic acid and acteoside in Plantaginis semen

被引：0

作者：

Rie Tanaka

Risa Inagaki

Naoki Sugimoto

Hiroshi Akiyama

Akito Nagatsu

机构：

[1] Kinjo Gakuin University,Laboratory of Pharmacognosy, College of Pharmacy

[2] National Institute of Health Sciences,undefined

来源：

Journal of Natural Medicines | 2017年 / 71卷

关键词：

Geniposidic acid; Acteoside; H-qNMR; Plantaginis semen; Reagent purity;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A quantitative 1H-NMR method (1H-qNMR) was developed to determine the concentration of acteoside and geniposidic acid in Plantaginis semen, which is an important crude drug for diuretic purposes. The purity of geniposidic acid and acteoside was determined by the ratio of the intensity of the H-3 signal at δ 7.51 ppm or the H-7″ signal at δ 7.58 ppm in methanol-d4 to that of a hexamethyldisilane (HMD) signal at 0.04 ppm, respectively. The concentration of HMD was corrected with the International System of Units traceability using potassium hydrogen phthalate of certified reference material grade. The geniposidic acid content in two batches of Plantaginis semen as determined by 1H-qNMR was found to be 0.84 and 1.00 %, and the acteoside content was determined to be 0.80 and 0.93 %. We demonstrated that this method is useful for the quantitative analysis of geniposidic acid and acteoside in Plantainis semen.

引用

页码：315 / 320

页数：5

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